Which of the following statements best describes a key difference in translation initiation between bacterial and eukaryotic cells?
Bacterial cells use a 5' cap to initiate translation, while eukaryotic cells use a Shine-Dalgarno sequence.
Bacterial cells use a Shine-Dalgarno sequence to direct ribosomes to the start codon, while eukaryotic cells use a 5' cap and scanning mechanism.
Eukaryotic cells require initiation factors (IFs) while bacteria do not
The start codon in bacteria is typically methionine, while in eukaryotes it is formylmethionine.
ปัญหา 2
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Which of the following statements best describes the attenuation process of the trp operon at high tryptophan concentrations?
The ribosome stalls at the leader sequence, allowing transcription of the entire operon.
The trp repressor binds to the operator, blocking transcription.
The leader sequence forms a terminator loop, preventing transcription of the structural genes.
Attenuation does not occur at high tryptophan concentrations.
ปัญหา 3
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Which histone modification typically increases gene expression?
Deacetylation
Phosphorylation
Acetylation
Methylation
ปัญหา 4
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During transcription, RNA polymerase synthesizes RNA in the:
5' → 3' direction using the 3' → 5' DNA template strand
3' → 5' direction using the 5' → 3' DNA template strand
5' → 3' direction using the 5' → 3' DNA template strand
3' → 5' direction using the 3' → 5' DNA template strand
ปัญหา 5
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Given the following coding DNA strand (with annotated regions):
What is the corresponding mRNA sequence?
5'-AUCGAUCGAUCGUACGUACG-3'
3'-AUCGAUCGAUCGUACGUACG-5'
5'-TAGCTAGCTAGCATGCATGC-3'
5'-UAGCUAGCUAGCAUGCAUGC-3'
ปัญหา 6
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How does aminoacyl-tRNA synthetase recognize the correct tRNA?
By reading the codon on the mRNA
By binding to the ribosomal A-site
Through anticodon-codon pairing
By recognizing structural features and the anticodon loop
ปัญหา 7
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Which ribosomal site does a new aminoacyl-tRNA enter during elongation?
E site
P site
A site
T site
ปัญหา 8
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Which of the following is NOT a stop codon (from 5' to 3' direction)?
UGA
UAG
UAA
AUG
ปัญหา 9
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Shown in the Figure is a diagram of high-resolution electron micrograph that illustrates some essential cellular processes. What does Structure A most likely represent in the electron micrograph?
DNA template strand
Ribosome
mRNA strand
Golgi apparatus
ปัญหา 10
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Shown in the Figure is a diagram of high-resolution electron micrograph that illustrates some essential cellular processes. What is Structure C, forming a growing chain?
DNA polymerase
Ribosomal large subunit
mRNA
Protein chain
ปัญหา 11
เลือกประเภท
What is Structure D, appearing as a larger molecular complex attached to mRNA (Structure E)?
tRNA
DNA repair complex
Ribosomal complex
Golgi vesicle
ปัญหา 12
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What label is appropriate for the left end (f) of structure A (DNA strand)?
N terminus
C terminus
3' end
5' end
ปัญหา 13
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What is the label for the beginning (i) of the polypeptide chain (structure C)?
Amino terminus (N-terminus)
C-terminus
5'-end
3'-end
ปัญหา 14
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Based on the diagram, which type of cell is this process most representative of?
Eukaryotic cell only
Prokaryotic cell only
Could be either prokaryotic or eukaryotic
Neither
ปัญหา 15
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Which feature would most help confirm that the diagram represents a prokaryotic cell rather than a eukaryotic one?
Presence of mRNA
A growing polypeptide
Translation occurring in cytoplasm, while transcription is still occurring.
5’ cap and poly-A tail on mRNA
ปัญหา 16
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Please design a pair of primers to amplify the full-length of the open reading frame (ORF; From start to stop codon) of this gene by PCR (The length of both primers is 20 base pair).
5’-ATGGCTAACAAAAAAGTAAC-3’ and 5'-TGCATTTCAGAAAGAGAAAATAA-3'
5'-TGCATTTCAGAAAGAGAAAATAA-3' and 5'-GTTACTTTTTTGTTAGCCAT-3'
5’-ATGGCTAACAAAAAAGTAAC-3’ and 5’TTATTTTCTCTTTCTGAAATGCAAT-3’
5’TTATTTTCTCTTTCTGAAATGCAAT-3’ and 5'-TGCATTTCAGAAAGAGAAAATAA-3'
ปัญหา 17
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Why can’t eukaryotic translation be coupled to transcription?
Eukaryotic ribosomes are inactive
DNA is in the cytoplasm
Transcription occurs in the nucleus, translation in the cytoplasm
D. Eukaryotes have no operons
ปัญหา 18
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Eukaryotic mRNAs are usually:
Polycistronic
Monocistronic
Circular DNA
Intronic-free
ปัญหา 19
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What is the purpose of the 5’ cap on eukaryotic mRNA?