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Cell and Molecular Biology

Plaipol Dedvis
127
ปัญหาที่เพิ่มเข้ามา (19/ 20)
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Cell and Molecular Biology Lecture_organized.pdf
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ปัญหา 1

เลือกประเภท

Which of the following statements best describes a key difference in translation initiation between bacterial and eukaryotic cells?

  • Bacterial cells use a 5' cap to initiate translation, while eukaryotic cells use a Shine-Dalgarno sequence.
  • Bacterial cells use a Shine-Dalgarno sequence to direct ribosomes to the start codon, while eukaryotic cells use a 5' cap and scanning mechanism.
  • Eukaryotic cells require initiation factors (IFs) while bacteria do not
  • The start codon in bacteria is typically methionine, while in eukaryotes it is formylmethionine.

ปัญหา 2

เลือกประเภท

Which of the following statements best describes the attenuation process of the trp operon at high tryptophan concentrations?

  • The ribosome stalls at the leader sequence, allowing transcription of the entire operon.
  • The trp repressor binds to the operator, blocking transcription.
  • The leader sequence forms a terminator loop, preventing transcription of the structural genes.
  • Attenuation does not occur at high tryptophan concentrations.

ปัญหา 3

เลือกประเภท

Which histone modification typically increases gene expression?

  • Deacetylation
  • Phosphorylation
  • Acetylation
  • Methylation

ปัญหา 4

เลือกประเภท

During transcription, RNA polymerase synthesizes RNA in the:

  • 5' → 3' direction using the 3' → 5' DNA template strand
  • 3' → 5' direction using the 5' → 3' DNA template strand
  • 5' → 3' direction using the 5' → 3' DNA template strand
  • 3' → 5' direction using the 3' → 5' DNA template strand

ปัญหา 5

เลือกประเภท

Given the following coding DNA strand (with annotated regions): What is the corresponding mRNA sequence?

  • 5'-AUCGAUCGAUCGUACGUACG-3'
  • 3'-AUCGAUCGAUCGUACGUACG-5'
  • 5'-TAGCTAGCTAGCATGCATGC-3'
  • 5'-UAGCUAGCUAGCAUGCAUGC-3'

ปัญหา 6

เลือกประเภท

How does aminoacyl-tRNA synthetase recognize the correct tRNA?

  • By reading the codon on the mRNA
  • By binding to the ribosomal A-site
  • Through anticodon-codon pairing
  • By recognizing structural features and the anticodon loop

ปัญหา 7

เลือกประเภท

Which ribosomal site does a new aminoacyl-tRNA enter during elongation?

  • E site
  • P site
  • A site
  • T site

ปัญหา 8

เลือกประเภท

Which of the following is NOT a stop codon (from 5' to 3' direction)?

  • UGA
  • UAG
  • UAA
  • AUG

ปัญหา 9

เลือกประเภท

Shown in the Figure is a diagram of high-resolution electron micrograph that illustrates some essential cellular processes. What does Structure A most likely represent in the electron micrograph?

  • DNA template strand
  • Ribosome
  • mRNA strand
  • Golgi apparatus

ปัญหา 10

เลือกประเภท

Shown in the Figure is a diagram of high-resolution electron micrograph that illustrates some essential cellular processes. What is Structure C, forming a growing chain?

  • DNA polymerase
  • Ribosomal large subunit
  • mRNA
  • Protein chain

ปัญหา 11

เลือกประเภท

What is Structure D, appearing as a larger molecular complex attached to mRNA (Structure E)?

  • tRNA
  • DNA repair complex
  • Ribosomal complex
  • Golgi vesicle

ปัญหา 12

เลือกประเภท

What label is appropriate for the left end (f) of structure A (DNA strand)?

  • N terminus
  • C terminus
  • 3' end
  • 5' end

ปัญหา 13

เลือกประเภท

What is the label for the beginning (i) of the polypeptide chain (structure C)?

  • Amino terminus (N-terminus)
  • C-terminus
  • 5'-end
  • 3'-end

ปัญหา 14

เลือกประเภท

Based on the diagram, which type of cell is this process most representative of?

  • Eukaryotic cell only
  • Prokaryotic cell only
  • Could be either prokaryotic or eukaryotic
  • Neither

ปัญหา 15

เลือกประเภท

Which feature would most help confirm that the diagram represents a prokaryotic cell rather than a eukaryotic one?

  • Presence of mRNA
  • A growing polypeptide
  • Translation occurring in cytoplasm, while transcription is still occurring.
  • 5’ cap and poly-A tail on mRNA

ปัญหา 16

เลือกประเภท

Please design a pair of primers to amplify the full-length of the open reading frame (ORF; From start to stop codon) of this gene by PCR (The length of both primers is 20 base pair).

  • 5’-ATGGCTAACAAAAAAGTAAC-3’ and 5'-TGCATTTCAGAAAGAGAAAATAA-3'
  • 5'-TGCATTTCAGAAAGAGAAAATAA-3' and 5'-GTTACTTTTTTGTTAGCCAT-3'
  • 5’-ATGGCTAACAAAAAAGTAAC-3’ and 5’TTATTTTCTCTTTCTGAAATGCAAT-3’
  • 5’TTATTTTCTCTTTCTGAAATGCAAT-3’ and 5'-TGCATTTCAGAAAGAGAAAATAA-3'

ปัญหา 17

เลือกประเภท

Why can’t eukaryotic translation be coupled to transcription?

  • Eukaryotic ribosomes are inactive
  • DNA is in the cytoplasm
  • Transcription occurs in the nucleus, translation in the cytoplasm
  • D. Eukaryotes have no operons

ปัญหา 18

เลือกประเภท

Eukaryotic mRNAs are usually:

  • Polycistronic
  • Monocistronic
  • Circular DNA
  • Intronic-free

ปัญหา 19

เลือกประเภท

What is the purpose of the 5’ cap on eukaryotic mRNA?

  • Marks the end of transcription
  • Initiates DNA replication
  • Assists ribosome binding
  • Targets the mRNA for degradation
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